真核生物磷酸盐转运蛋白的晶体结构

研究人员报告了一个真菌高亲和性磷酸盐转运蛋白PiPT在磷酸盐存在时的X-射线晶体结构，它处在一种朝向里面的、被阻隔的状态。该结构可以解释该转运蛋白对于磷酸盐亲和性和结合特异性的机制，并将质子动力与磷酸盐转位了起来。

PiPT磷酸转运蛋白的晶体结构为那些与癌症和糖尿病等疾病有关的关键转运蛋白等提供了一个有用模型。

Crystal structure of a eukaryotic phosphate transporter

Bjørn P. Pedersen, Hemant Kumar, Andrew B. Waight, Aaron J. Risenmay, Zygy Roe-Zurz,Bryant H. Chau, Avner Schlessinger, Massimiliano Bonomi, William Harries, Andrej Sali,Atul K. Johri & Robert M. Stroud

Phosphate is crucial for structural and metabolic needs, including nucleotide and lipid synthesis, signalling and chemical energy storage. Proton-coupled transporters of the major facilitator superfamily （MFS） are essential for phosphate uptake in plants and fungi, and also have a function in sensing external phosphate levels as transceptors1, 2, 3, 4, 5. Here we report the 2.9 Å structure of a fungal （Piriformospora indica） high-affinity phosphate transporter, PiPT, in an inward-facing occluded state,